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Zhang,
Yong (Cellular &
Molecular Bioengineering)
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Zhang, Yong (Ph.D.) Associate
Professor E1-05-18, Division of Bioengineering Faculty of
BN4404 BioMEMS BN3303 Introduction to Biomaterials BN1901 Principles of Bioengineering GEK2505 Introductory Biomedical Engineering |
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Research Projects (1) Fluorescence based biodetection &
bioimaging Both down-conversion and up-conversion
fluorescent inorganic nanoparticles (quantum dots, lanthanide doped
nanocrystals) are synthesized and used as fluorescent labels or imaging
probes for biodetection & bioimaging. The up-conversion fluorescent
nanoparticles can convert near infrared (NIR) light to visible light.
Compared to conventional down-conversion fluorescent materials such as
organic dyes and quantum dots, these nanoparticles have the following
advantages: High light penetration depth in tissues; No photodamage to living
organisms; Weak autofluorescence from cells or tissues; Low background light
and high sensitivity for detection.
Li ZQ & Zhang Y, Angewandte Chemie
International Edition 2006, 45,
7732-7735. (2) Imaging-guided cancer therapy Multi-color
fluorescent quantum dots and magnetic agents are encapsulated within
nanometer-sized (~50 nm) chitosan nanoparticles. The small size of the
nanoparticles allows them to be used as a labeling tag, at the same time, as
a contrast agent in magnetic resonance imaging (MRI) as well. In the labeling
of cancer cells, specific targeting molecules that recognize cancer cells can
be attached to the surface of the nanoparticles so that they bind onto the
surface of the cancer cells specifically. This can potentially help in the
localization and identification of a cancerous tissue. Moreover, these
nanoparticles can be used to deliver therapeutic drugs, proteins and genes by
intravenous, oral and mucosal administration. Using these nanoparticles,
drugs or genes can be precisely delivered to the specific cells or specific
regions of tissues with aid of imaging techniques, for various applications.
Tan WB & Zhang Y, Advanced Materials 2005, 17, 2375-2380. Tan WB, Jiang S & Zhang Y. Biomaterials, in press (3) Bead
based microarrays for multiplexing bioassays Compared to the microarrays fabricated on planar substrates, bead
based microarrays are more robust as microbeads are ideal reagent delivery
vehicles providing large reactive surface areas and have become omnipresent
in biomedical applications. A technique is developed to fabricate a
microfluidic device with unique dome-shape structures for high efficiency
immobilization and patterning of single microbeads. We have also fabricated
polymer porous films with tunable pore sizes by employing non-lithographic
“breadth figure” method and colloidal template method, for
patterning of microbeads. Our research aims to use arrays of encoded
microbeads for high-throughput multiplexing bioassays.
Lu MH & Zhang Y, Advanced Materials
2006, 18, 3094-3098. (4) Micropatterning
of proteins & cells via self-assembled nanoparticles Micropatterning of biomolecules
forms the basis of cell culture, biosensor and microarray technology. We have
reported methods to pattern biomolecules through self-assembling polystyrene
nanoparticles in arrayed microwells on a solid surface to form well-ordered
patterning, followed by attaching biomolecules and cells to the assembled
nanoparticles.
Wang C & Zhang Y, Advanced Materials
2005, 17,
150-153. Yap FL & Zhang Y, Langmuir 2005, 21, 5233-5236. |
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