CJR Sheppard Microscope Imaging

04/20/04

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4. Microscope imaging

4.1 Microscope imaging

4.2 Signal, noise and detectors

 

 

4.1 Microscope imaging

C. 2. Sheppard CJR, Wilson T, Gannaway JN (1978) Image formation in scanning microscopes, ICO -11, Madrid, September 1978, in Optica Hoy y Mañana, J Bescos, A Hidalgo, L Plaza, J Santamaria, eds. Sociedad Española de Optica, Madrid, pp. 211-214. ISBN 84 600 1317 0

 

D. 8. Sheppard CJR (1977) The use of lenses with annular aperture in scanning optical microscopy, Optik 48, 329-334.

D. 10. Sheppard CJR,  Choudhury A (1977) Image formation in the scanning microscope, Optica Acta 24, 1051-1073.

 

First published theory of confocal microscopy.

 

First paper to use the term "confocal microscope":

 

“It is shown that there are two geometries of the (scanning) microscope, which have been designated Type 1 and Type2. Those of Type 1 exhibit identical imaging to the conventional microscope, whereas those of Type 2 (confocal microscopes) display various differences. ... It is found that Type 2 microscopes have improved imaging properties over conventional microscopes and that these may be further improved by use of one or two lenses with annular pupils.”

New Oxford Dictionary of English:

confocal microscope:

“denoting or using a microscope whose imaging system only collects light

from a small spot on the specimen, giving greater resolution.”

 

D. 12. Sheppard CJR, Wilson T  (1978) Image formation in scanning microscopes with partially coherent source and detector, Optica Acta  25, 315-325.

 

D. 16. Sheppard CJR, Choudhury A (1978) The image of a bar-pattern in the scanning microscope, Optik 51, 361-368.

 

D. 17. Sheppard CJR, Wilson T (1978) Depth of field in the scanning microscope, Optics Letts. 3, 115-117.

D. 20. Sheppard CJR, Wilson T (1978) The theory of scanning microscopes with Gaussian pupil functions, J. Microscopy 114, 179-197.

D. 24. Sheppard CJR, Wilson T (1979) Imaging properties of annular lenses, Appl. Opt. 18, 3764-3769.

D. 30. Sheppard CJR, Wilson T (1980) Image formation in confocal scanning microscopes, Optik 55, 331-342.

D. 31.  Sheppard CJR, Wilson T (1980) Multiple traversing of the object in the scanning microscope, Optica Acta 27, 611-624.

E.9. Sheppard CJR, Wilson T (1980) Image formation in confocal optical systems, Int. Optical Computing Conference, Washington DC, Proc. SPIE 232, 197-202.

D. 34. Sheppard CJR, Wilson T (1981) The theory of the direct-view confocal microscope, J. Microsc. 124, 107-117.

 

D. 39. Cox IJ, Sheppard CJR, Wilson T (1982) Improvement in resolution by nearly confocal microscopy, Appl. Opt. 21, 778-781.

D. 40. Cox IJ, Sheppard CJR, Wilson T (1982) Super-resolution by confocal fluorescent microscopy, Optik 60, 391-396.

 

Reproduced in SPIE Milestone Series MS-131, Selected reprints on Confocal microscopy, BR Masters, ed. ISBN 0-8194-2376-6, pp 178-181

 

D. 45. Sheppard CJR, Hamilton DK, Cox, IJ (1983) Optical microscopy with extended depth of field, Proc. R. Soc. Lond. A 387, 171-186.

 

“A method is described that allows depth of field in optical microscopy to be extended, in principle without limit, while high-resolution, diffraction-limited imaging is retained. Experimentally, an extension of more than two orders of magnitude has been achieved. The images are found to be of high optical quality, and this is born out by theoretical predictions. The resolution is shown theoretically to be similar to that in a conventional microscope of the same numerical aperture, and for some criteria an improvement is obtained.”

 

Presents the defocused coherent transfer function for a confocal reflection microscope.

 

D. 50. Sheppard CJR (1984) Intensity at the edge in the image of a straight edge with full illumination, Optik  66,  191-194.

 

D. 51. Sheppard CJR, Cox IJ, Hamilton DK (1984) Edge detection in micrometrology with nearly confocal microscopy, Appl. Opt.  23, 657-658.

D. 52. Sheppard CJR, Hamilton DK (1984) Edge enhancement by defocusing of confocal images, Optica Acta  31,  723-727.

D. 53. Sheppard CJR (1984) Optical resolution and the spatial frequency cut-off, Optik 66, 311-315.

E.14. Sheppard CJR, Cox IJ (1985) Resolution of scanned optical systems, Image Science '85, Helsinki June 1985, Acta Polytechnica Scandinavica, Appl. Phys. Ser. 149, 279-281.

D. 62. Sheppard CJR, Wilson,T (1986) On the equivalence of scanning and conventional microscopes, Optik 78, 39-43.

 

D. 65. Sheppard CJR Wilson T (1986) Reciprocity and equivalence in scanning microscopes, J. Opt. Soc. Am. 3, 755-756.

 

E.16. Sheppard CJR (1986) Optical sectioning in confocal microscopy, 19 Kolloquium Arbeitkreises für Elektronenmikroskopische Direktabbildung und Analyse von Oberflächen, Aachen, 1986, Beitr. elektronenmikroskop., Direktabb., Oberfl. 19, 133-138.

 

D. 81. Sheppard CJR (1988) Depth of field in optical microscopy, J. Microsc. 149, 73-75.

 

D. 88. Sheppard CJR (1989) Axial resolution of confocal fluorescence microscopy,
J. Microsc. 154, 237-241.

 

D. 98. Sheppard CJR, Gu M  (1991) Optical sectioning in confocal microscopes with annular pupil, Optik 86, 169-172.

D. 114. Sheppard CJR, Gu M (1991) Improvement of axial resolution in confocal microscopy using an annular pupil, Opt. Commun. 84, 7-13.

D. 125. Sheppard CJR, Gu M (1992) The edge-setting criterion in confocal microscopy.  Appl. Opt. 31, 4575-4577.

D. 127. Gu M, Sheppard CJR (1992) Effects of annular pupils on confocal fluorescent imaging,  J. Mod. Opt. 39, 1883-1896.

D. 128. Wilson T, Hewlett SJ and Sheppard CJR (1990) Use of objective lenses with slit pupil functions in the imaging of line structures, Appl. Opt. 29, 4705-4714.

D. 134. Gu M, Sheppard CJR, Zhou H (1993) Optimization of axial resolution in confocal imaging using annular  pupils,  Optik 93, 87-90.

D. 140. Gu M, Sheppard CJR (1994) Effects of defocus and primary spherical aberration on images of a straight edge in confocal microscopy, Applied Optics 33, 625-630.

D. 152. Gan XS, Sheppard CJR (1993) Imaging in a confocal microscope with one circular and one annular lens, Opt. Commun.103, 254-264.

D. 156. Gu M, Tannous T, Sheppard CJR (1994) Improved axial resolution in confocal fluorecence microscopy using annular pupils,  Opt. Commun. 110, 533-539.

D. 157. Sheppard CJR, Gu M (1994) Imaging performance of confocal fluorescence microscopes with finite-sized source  J. Mod. Opt. 41, 1521-1530.

D. 159. Zhou H, Gu M, Sheppard CJR (1995) Investigation of aberration measurement in confocal microscopy J. Mod. Opt., 42,627-638

D. 160. Zhou H, Gu M, Sheppard CJR (1994) Confocal interference microscopy:  a difference between dry and immersion lenses, Optik 97, 94-100.

D. 173. Sheppard CJR (1995) Transport equations for confocal imaging, Optik, 11, 141-142

D. 177. Gu M, Tannous T, Sheppard CJR (1996) Effect of numerical aperture and annular pupil on confocal imaging through highly-scattering media  Opt. Lett..21, 312-314

D. 235. Sheppard CJR, Aguilar JF (2000) Electromagnetic imaging in the confocal microscope, Opt. Commun. 180, 1-8

D. 249. Sheppard CJR, Sharma MD Integrated intensity, and imaging through scattering media, J. Mod. Opt., 48, 1517-1525

D. 268. Cox G, Sheppard CJR (2004) Practical limits of resolution in confocal and non-linear microscopy, Microscopy Research and Technique, 63, 18-22

 

4.2 Signal, noise and detectors

D. 104. Sheppard CJR, Cogswell CJ, Gu M (1991) Signal strength and noise in confocal microscopy: factors influencing selection of an optimum detector aperture,  Scanning 13, 233-240.

D. 116. Sheppard CJR (1991) Stray light and noise in confocal microscopy, Micron and Microscopia Acta  22, 239-243.

E.30. Sheppard CJR, Gu M, Roy M (1992) Signal strength and its effects on processing of confocal images, Proc. SPIE 1660, 243-249

D.131. Sheppard CJR, Gu M, Roy M (1992) Signal to noise ratio in confocal microscope systems,  J.Microscopy, 168, 209-218.

D. 151. Gan XS and Sheppard CJR (1993) Detectability: A new criterion for evaluation of the confocal microscope, Scanning 15, 187-192.

D. 153. Roy M, Sheppard CJR (1993) Effect of image processing on the noise properties of confocal imaging, Micron 24, 623-635.

C. 23. Sheppard CJR, Gan XS, Gu M, Roy M (1995) Noise in Confocal Microscopes, in The Handbook of Biological Confocal Microscopy, J. Pawley,ed. Plenum Press, New York, pp. 363-371. ISBN 0 306 44826 2

D. 230. Gauderon R, Sheppard CJR (1999) Effect of a finite-sized pinhole on noise performance in single-, two-, and three-photon confocal fluorescence microscopy, Appl. Opt. 38, 3562-3565

 

 

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This site was last updated 04/20/04