HUANG Zhiwei

  • Associate Professor

Research Interest

  1. Biomedical Optical Spectroscopy (Raman, Fluorescence and Diffuse Reflectance) and Imaging in Biomedicine
  2. Advanced Fiber-Optic Endoscopic Imaging
  3. Coherent Raman Scattering Microscopy Imaging
  4. Multimodal Nonlinear Optical Microscopy Imaging
  5. Nonlinear Fluorescence Lifetime Imaging

Teaching Modules

  • BN2101 Principles of Bioengineering
  • BN2203 Introduction to Bioengineering Design
  • BN3401 Biomedical Electronics and Systems
  • BN4406 Biophotonics and Bioimaging
  • GEK2505 Introductory Biomedical Engineering
  • BN5207 Medical Imaging Systems

Selected Journal Publications

  1. K Bae, W Zheng, and Z Huang*, Quantitative assessment of spinal cord injury using circularly polarized coherent anti-Stokes Raman scattering microscopy, Appl. Phy. Lett., 111, 063704 (2017); doi: 10.1063/1.4991792.
  2. K Lin, W Zheng, C Lim, Z Huang*, Real-time in vivo diagnosis of nasopharyngeal carcinoma using rapid fiber-optic Raman spectroscopy, Theranostics, 7(14):3517-3526 (2017). doi:10.7150/thno.16359.
  3. Y Ye, F Xu, G Wei, Y Xu, D Pu, L Chen, Z Huang*, Scalable Fourier transform system for instantly structured illumination in lithography, Optics Letters, 42(10), 1978- 1981 (2017).
  4. C Lim, R Nagaraja, T Loh, W Zheng, J Wang, K Lin, Z Huang*, Real time near-infrared Raman spectroscopy for the diagnosis of nasopharyngeal cancer, Oncotarget, 2017, 1-8, doi: 10.18632/oncotarget.17703.
  5. K Lin, W Zheng Wei, CM Lim, Z Huang*, Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman endoscopy, Biomedical Optics Express, 7 (9), 3705-3715 (2016).
  6. Z Wang, W Zheng, Z Huang*, Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging, Optics Letters, 41 (17), 3960-3963 (2016).
  7. J Wang, W Zheng, K Lin, Z Huang*, Development of a hybrid Raman spectroscopy and optical coherence tomography technique for real-time in vivo tissue measurements, Optics Letters, 41 (13), 3045-3048 (2016).
  8. K Lin, J Wang, W Zheng, K Ho, M Teh, K Yeoh, Z Huang*, Rapid fiber-optic Raman spectroscopy for real-time in vivo detection of gastric intestinal metaplasia during clinical gastroscopy, Cancer Prevention Research, 2016; doi: 10.1158/1940-6207.CAPR-15-0213.
  9. J Wang, W Zheng, K Lin, and Z Huang*, Integrated Mueller-Matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection, Biomedical Optics Express, 7 (4), 1116-1126 (2016). doi: 10.1364/BOE.7.001116.
  10. Z Wang, W Zheng, CY Hsu, and Z Huang*, Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy, Biomedical Optics Express, 7(4), 1284-1293 (2016). DOI:10.1364/BOE.7.001284.
  11. Z Wang, W Zheng, CY Hsu, and Z Huang*, Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth, Appl. Phys. Lett., 108, 033701 (2016); doi: 10.1063/1.4939923.
  12. J Wang, K Lin, W Zheng, K Ho, M Teh, K Yeoh, Z Huang*, Fiber-optic Raman spectroscopy for in vivo diagnosis of gastric dysplasia, Faraday Discuss., 2015, DOI: 10.1039/C5FD00151J
  13. Z Dong, M Asbahi, J Lin, D Zhu, Y Wang, K Hippalgaonkar, H Chu, W Goh, F Wang, Z Huang*, and J Yang*, Second-Harmonic Generation from Sub-5 nm Gaps by Directed Self-Assembly of Nanoparticles onto Template-Stripped Gold Substrates, Nano Lett., 2015. DOI: 10.1021/acs.nanolett.5b02109.
  14. J Wang, K Lin, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy, Scientific Reports, 5, 12957; DOI: 10.1038/srep12957 (2015).
  15. J Wang, K Lin, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Comp arative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy. Anal. Bioanal. Chem., (2015); DOI:10.1007/s00216-015-8727-x.
  16. M Bergholt, K Lin, J Wang, W Zheng, H Xu, Q Huang, JL Ren, KY Ho, M Teh, S Srivastava, B Wong, KG Yeoh, Z Huang* , Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy. J. Biophotonics,(2015); DOI: 10-1002/jbio.201400141.
  17. Z Wang, W Zheng, CY Hsu, and Z Huang*, Epi-detected quadruple-modal nonlinear optical microscopy for label-free tooth imaging, Appl. Phys. Lett., 106, 033701 (2015).
  18. M Bergholt, W Zheng, K Lin, J Wang, H Xu, J Ren, K Ho, M Teh, K Yeoh, Z Huang*, Characterizing variability of in vivo Raman spectroscopic properties of different anatomical sites of normal colorectal tissue towards cancer diagnosis at colonoscopy", Anal. Chem., 87, 960-966 (2015).
  19. J Lin, W Zheng, Z Wang, and Z Huang*, Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy, Appl. Phys. Lett. 105, 103705 (2014).
  20. S. Duraipandian, J Mo, W. Zheng, and Z. Huang*, "Near-infrared Raman spectroscopy for assessing biochemical changes of cervical tissue associated with precarcinogenic transformation" Analyst, 139 (21), 5379-5386 (2014).
  21. J Lin, SK Teh, W Zheng, Z Wang, Z Huang*, Multimodal nonlinear optical microscopic imaging provides new insights into acetowhitening mechanisms in live mammalian cells without labelling, Biomedical Optics Express, 5(9), 3116-3122 (2014).
  22. M Bergholt, W Zheng, KY Ho, M Teh,  KG Yeoh, J So, A Shabbir, Z Huang*, Fiber-optic confocal Raman spectroscopy for real-time in vivo diagnosis of dysplasia in Barrett's esophagus,  Gastroenterology, 146(1), 27-32 (2014).
  23. M Bergholt, S Duraipandian, W Zheng, Z Huang*, Multivariate reference technique for quantitative analysis of fiber-optic tissue Raman spectroscopy, Anal. Chem. 85, 11297-11303 (2013).
  24. J Lin, S Pan, W Zheng, Z Huang*, Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling, Appl. Phys. Lett. 103, 173701 (2013).
  25. M Bergholt, W Zheng, KY Ho, M Teh,  KG Yeoh, J So, A Shabbir, Z Huang*, Raman endoscopy for objective diagnosis of early cancer in the gastrointestinal system, Journal of Gastrointestinal & Digestive System, 2013, S:1. http://dx.doi.org/10.4172/2161-069X.S1-008.
  26. J Lin, K Er, W Zheng, Z Huang*,  Radially polarized tip-enhanced near-field coherent anti-Stokes Raman scattering microscopy for vibrational nano-imaging, Appl. Phys. Lett., 103, 083705 (2013)
  27. J Wang, M Bergholt, W Zheng, Z Huang*, Development of a beveled fiber-optic confocal Raman endoscopic probe for enhancing in vivo epithelial tissue Raman measurements at endoscopy. Optics Letters, 38 (13), 2321-2323 (2013).
  28. S Duraipandian, W Zheng, J Ng, J.H. Low, A. Ilancheran, and Z Huang*, Near-infrared-excited confocal Raman spectroscopy advances in vivo detection of cervical precancer, J. Biomed. Opt. 18 (6), 067007 (2013).
  29. S Duraipandian, W Zheng, J Ng, J.H. Low, A. Ilancheran, and Z Huang*, Noninvasive analysis of hormonal variations and vagifem treatment effect on postmenopausal women using in vivo high wavenumber confocal Raman spectroscopy, Analyst, 138 (14), 4120-4128 (2013).
  30. P Upputuri, J Lin, G Li, X Liu, H Wang*, Z Huang*,  Circularly Polarized Coherent Anti-Stokes Raman Scattering Microscopy,  Optics Letters, 38(8),  1262-1264 (2013).
  31. M Bergholt, W Zheng, Z Huang*, Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy, Journal of Biomedical Optics, 18 (3), 30502 (2013).
  32. M Bergholt, W Zheng, KY Ho, M Teh, KG Yeoh, J So, A Shabbir, Z Huang*, Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy, J. Biophotonics Vol.6, No.1, 49-59 (2013) DOI 10.1002/jbio.201200138.
  33. Men-Qiao Dai, Wei Zheng, Zhiwei Huang and Lin-Yue Lanry Yung, Aqueous phase synthesis of widely tunable photoluminescence emission CdTe/CdS core/shell quantum dots under a totally ambient atmosphere, J. Mater. Chem. 22 (32), 16336 - 16345 (2012)
  34. M Bergholt, W Zheng, Z Huang*, Characterizing variability in in vivo Raman spectroscopic properties of different anatomical sites of normal tissue in the oral cavity, Journal of Raman Spectroscopy, 43, 255-262 (2012).
  35. K Lin, D Lau, Z Huang*, Optical diagnosis of laryngeal cancer using high wavenumber Raman spectroscopy, Biosensors and Bioelectronics 35, 213-217 (2012).
  36. S Duraipandian, W Zheng, J Ng, Jeffrey Low, A Ilanacheran, Z Huang*, 
Simultaneous fingerprint and high wavenumber confocal Raman spectroscopy enhances early detection of cervical precancer in vivo, Analytical Chemistry, 84, 5913-5919 (2012).
  37. M Bergholt, K Lin, W Zheng, D Lau, Z Huang*, In vivo real-time transnasal image-guided Raman endoscopy: defining spectral properties in the nasopharynx and larynx, Journal of Biomedical Optics, 17 (7), 077002 (2012).
  38. S Duraipandian, M Bergholt, W Zheng, K Ho, M Teh, K Yeoh, J So, A Shabbir, Z Huang*, Real-time biomedical Raman spectroscopy for in vivo, on-line gastric cancer diagnosis during clinical endoscopic examination, Journal of Biomedical Optics, 17 (8), 081418 (2012).
  39. 6. J Lin , F Lu , W Zheng , S Xu , D Tai , H Yu , Z Huang*, Assessment of liver steatosis and fibrosis in a bile duct ligation rat model using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique, Journal of Biomedical Optics, 16(11), 116024 (2011).
  40. X Shao, W Zheng, Z Huang*, Near-infrared autofluorescence spectroscopy for in vivo identification of hyperplastic and adenomatous polyps in the colon, Biosensors and Bioelectronics, 30, 118-122(2011).
  41. S Duraipandian, W Zheng, J Ng, Jeffrey Low, A Ilanacheran, Z Huang*, In vivo diagnosis of cervical precancer using Raman spectroscopy and genetic algorithm techniques, Analyst, 136, 4328-4336 (2011).
  42. B Lin, M Bergholt, D Lau, Z Huang*, Optical diagnosis of nasopharyngeal cancer using ultraviolet autofluorescence excitation-emission matrix spectroscopy and parallel factor analysis, Analyst, 136, 3896-3903 (2011).
  43. X Shao, W Zheng, Z Huang*, In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling, Journal of Biomedical Optics, 16(6), 067005 (2011).
  44. M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer, Biosensors and Bioelectronics, 26, 4104-4110 (2011).
  45. M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, In vivo diagnosis of esophageal cancer using image-guided Raman endoscopy and biomolecular modeling, Technology in Cancer Research & Treatment (TCRT), 10 (2), 103-112 (2011).
  46. M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Characterizing variability in in vivo Raman spectra of different anatomical sites in the upper gastrointestinal tract toward cancer detection, Journal of Biomedical Optics, 16(3), 037003 (2011).
  47. N Lim, Z Hamed, C Yeow, C Chan, Z Huang*, Early detection of biomolecular changes in disrupted porcine cartilage using polarized Raman spectroscopy, Journal of Biomedical Optics, 16 (1), 017003 (2011).
  48. M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques, Int J Cancer, 128(11), 2673-2680 (2011). 
  49. X Shao, W Zheng, Z Huang*, Polarized near-infrared autofluorescence imaging combined with near-infrared diffuse reflectance imaging for improving colonic cancer detection, Optics Express, 18 (23), 24293-24300 (2010).
  50. M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Raman endoscopy for in vivo differentiation between benign and malignant ulcers in the stomach, Analyst, 135 (12), 3162–3168 (2010).
  51. Lin, F Lu, W Zheng, Z Huang*, Annular aperture-detected coherent anti-Stokes Raman scattering microscopy for high contrast vibrational imaging, Applied Physics Letters, 97 (8), 083701 (2010).
  52. C Yuen, W Zheng, Z Huang*, Low-level detection of anti-cancer drug in blood plasma using microwave-treated gold-polystyrene beads as surface-enhanced Raman scattering substrates, Biosensors and Bioelectronics, 26, 580-584 (2010).
  53. Z Huang*, SK Teh, W Zheng, K Lin, KY Ho, M Teh, KG Yeoh, In vivo detection of epithelial neoplasia in the stomach using image-guided Raman endoscopy, Biosensors and Bioelectronics, 26, 383-389 (2010).
  54. K Lin, W Zheng, Z Huang*, Integrated autofluorescence endoscopic imaging and point-wise spectroscopy for real-time in vivo tissue measurements, Journal of Biomedical Optics, 15 (4), 040507 (2010).
  55. C Fang, F Lu, W Zheng, Z Huang*, Triple-frequency symmetric subtraction scheme for nonresonant background suppression in coherent anti-Stokes Raman scattering (CARS) microscopy, Optics Express, 18 (15), 15714-15724 (2010).
  56. J Mo, W Zheng, Z Huang*, Fiber-optic Raman probe couples ball lens for depth-selected Raman measurements of epithelial tissue, Biomedical Optics Express, 1 (1), 17-30 (2010).
  57. Z Huang*, M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy, Journal of Biomedical Optics, 15 (3), 037017 (2010).
  58. J Lin, W Zheng, H Wang, Z Huang*, Effects of scatterers' sizes on near-field coherent anti-Stokes Raman scattering under tightly focused radially and linearly polarized light excitation, Optics Express, 18(10), 10888-10895 (2010).
  59. F Lu, W Zheng, J Lin, Z Huang*, Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser, Applied Physics Letters, 96(14), 133701 (2010)
  60. H Li, W Zheng, Z Huang*, Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophores characterization using an acousto-optic tunable filter technique, Journal of Biomedical Optics, 15 (2), 027010 (2010).
  61. SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for early diagnosis and typing of adenocarcinoma in the stomach, British Journal of Surgery, 97(4), 550-557 (2010).
  62. SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for optical diagnosis in the stomach: Identification of Helicobacter-pylori infection and intestinal metaplasia, Int. J. Cancer, 126 (8), 1920-1927 (2010).
  63. C Yuen, W Zheng, Z Huang*, Optimization of extinction efficiency of gold-coated polystyrene beads substrates improves surface-enhanced Raman scattering effects by post-growth microwave heating treatment, J. Raman Spectroscopy, 41 (4), 374-380 (2010).
  64. J Mo, W Zheng, Jeffrey Low, J Ng, A Ilanacheran, Z Huang*, High wavenumber Raman spectroscopy for in vivo detection of cervical dysplasia, Analytical Chemistry, 81, 8908-8915 (2009).
  65. J Lin, F Lu, H Wang, W Zheng, C Sheppard, Z Huang*, Improved contrast radially polarized coherent anti-Stokes Raman scattering microscopy using annular aperture detection, Applied Physics Letters, Vol. 95, No. 13, 133703 (2009).
  66. F Lu, W Zheng, Z Huang*, Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light, Optics Letters, Vol.34, No.12, 1870-1872 (2009).
  67. Z Huang*, SK Teh, W Zheng, J Mo, K Lin, X Shao, KY Ho, M Teh, KG Yeoh, Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy, Optics Letters, Vol.34, No.6, 758-760 (2009).
  68. J Lin, H Wang, W Zheng, F Lu, C Sheppard, Z Huang*, Numerical study of effects of light polarization, scatterer sizes and orientations on near-field coherent anti-Stokes Raman scattering microscopy, Optics Express, 17 (4), 2423-2434 (2009).
  69. SK Teh, W Zheng, David P Lau, Z Huang*, Spectroscopic diagnosis of laryngeal carcinoma using near-infrared Raman spectroscopy and random recursive partitioning ensemble techniques, Analyst, 134, 1232-1239 (2009).
  70. SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for gastric precancer diagnosis, J. Raman Spectroscopy, 40, 908-914 (2009).
  71. F Lu, W Zheng, Z Huang*, Elliptically polarized coherent anti-Stokes Raman scattering microscopy, Optics Letters, 33 (23), 2842-2844 (2008).
  72. F Lu, W Zheng, Z Huang*, Phase-controlled polarization coherent anti-Stokes Raman scattering microscopy for high sensitive and high contrast molecular imaging, Journal of the Optical Society of America B, 25 (11), 1907-1913 (2008).
  73. C Yuen, W Zheng, Z Huang*, Improving surface-enhanced Raman scattering effect using gold-coated hierarchical polystyrene bead substrates modified with post-growth microwave treatment, Journal of Biomedical Optics, 13 (6), 064040 (2008).
  74. C Yuen, W Zheng, and Z Huang*, Surface-enhanced Raman scattering: principles, nanostructures, fabrications and biomedical applications, J. Innovative Optical Health Sciences (JIOHS), 1 (2), 267-284 (2008).
  75. F. Jaillon, W. Zheng, and Z Huang*, Half-ball lens couples a beveled fiber probe for depth-resolved spectroscopy: Monte Carlo simulations, Appl. Opt. 47, 3152-3157 (2008).
  76. F Lu, W Zheng, and Z Huang*, Heterodyne polarization coherent anti-Stokes Raman scattering microscopy, Applied Physics Letters, 92 (12), 123901 (2008).
  77. F Lu, W Zheng, C Sheppard, Z Huang*, Interferometric polarization coherent anti-Stokes Raman scattering microscopy, Optics Letters, 33(6), 602-604 (2008).
  78. F Jaillon W Zheng, Z Huang*, Beveled fiber-optic probe couples a ball lens for improving depth-resolved fluorescence measurements of layered tissue: Monte Carlo Simulations, Phys. Med. Biol. 53, 937-951 (2008).
  79. SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Diagnostic potential of near-infrared Raman spectroscopy in the stomach: differentiating dysplasia from normal tissue. British Journal of Cancer, 98, 457-465 (2008).
  80. SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Diagnosis of gastric cancer using near-infrared Raman spectroscopy and classification and regression tree techniques, Journal of Biomedical Optics, 13 (3), 034013 (2008).
  81. E Widjaja, W Zheng and Z Huang*, Classification of colonic tissues using near-infrared Raman spectroscopy and support vector machines. Int. J. Oncology, 32: 653-662 (2008).