• AFM imaging of malaria infected cells.
  • Malaria infected red blood cells adhering and rolling on ICAM-1 coated surface.
  • Micropipette aspiration of a malaria infected red blood cell.
  • Dual pipette assay of cell-cell adhesion of malaria infected red blood cells.
  • Squeezing of a breast cancer cell (MCF-7 with stained nucleus) into a microfluidic channel (10x10um).
  • Collective migration of epithelial cells (Nuclei labeled with DAPI).
  • MDCK cells are unable to close a 100 μm diameter non-adhesive gap. Scale bar 50 μm. (SRK Vedula et al, Nature Comm, 2015).
  • a-catenin knockdown HaCaT cells are unable to close a 100 μm diameter non-adhesive gap. Scale bar 50 μm. (SRK Vedula et al, Nature Comm, 2015).
  • HaCaT cells closing a 100 μm diameter non-adhesive gap. Scale bar 50 μm. (SRK Vedula et al, Nature Comm, 2015).
  • HaCaT cells partially closing a 150 μm diameter non-adhesive gap. Scale bar 50 μm. (SRK Vedula et al, Nature Comm, 2015).
  • HaCaT cells unable to close a 200 μm diameter non-adhesive gap. Scale bar 50 μm. (SRK Vedula et al, Nature Comm, 2015).
  • MDCK epithelial cells do not form well-developed epithelial bridges but some ‘nascent’ bridge-like structures can be observed at the junction of the fibronectin strip and the reservoir. Scale bar 50 μm, Time stamp (hours: minutes). (SRK Vedula et al, Nature Materials, 2014).
  • Keratinocytes migrating from the reservoir (RES) into the 10 μm wide fibronectin strips form multicellular suspended epithelial bridges over the non-adhesive regions between the fibronectin strips. Scale bar 50 μm, Time stamp (hours: minutes). (SRK Vedula et al, Nature Materials, 2014).
  • Migration of HaCaT cells on 10 μm micropatterns in the presence of 50 μM blebbistatin. Despite cells migrating into the fibronectin strips, epithelial-bridge formation was markedly inhibited, suggesting that myosin contractility was indeed required for the formation of epithelial bridges. Scale bar 50 μm, Time stamp (hours: minutes). (SRK Vedula et al, Nature Materials, 2014).
  • Epithelial bridges collapse completely in presence of calyculin A 20 nM. Rupture starts at concave leading front. Scale bar 50 μm, Time stamp (minutes). (SRK Vedula et al, Nature Materials, 2014).
  • Laser ablation of the leading-cell front on the fibronectin strip results in immediate elastic recoil of the epithelial bridge, confirming their predominantly elastic nature as well as the existence of tension within them. (SRK Vedula et al, Nature Materials, 2014).
  • Radial flow of actin filaments on soft substrate. A REF52 cell, transiently expressing RFP-ftractin (labels F-actin), adhered to a soft substrate (9 nN/μm). (M Gupta et al, Nature Comm, 2015).
  • MCF-10A cells (benign breast cancer cells) migrating on 200 μm fibronectin patterns. Cells closer to the edge of the pattern show a collective behavior but those in the centre migrate randomly. Time stamp shows hours: minutes. (K Doxzen et al, Integrative Biology, 2013).
  • MCF-7 cells (invasive breast cancer cells) migrating on 200 μm fibronectin patterns showing little tendency for collective behavior. Time stamp shows hours: minutes. (K Doxzen et al, Integrative Biology, 2013).
  • MDA-MB-231 cells (invasive breast cancer cells) migrating on 200 μm fibronectin patterns showing little tendency for collective behavior. Time stamp shows hours: minutes.(K Doxzen et al, Integrative Biology, 2013).
  • Confluent MDCK cells exhibiting synchronized rotation on a 200 μm diameter fibronectin pattern. Time stamp shows hours: minutes. (K Doxzen et al, Integrative Biology, 2013).
  • MDCK cells migrating on a 500 μm diameter fibronectin pattern showing a localized ‘vortex’ ~ 300 μm in diameter. Time stamp shows hours: minutes. (K Doxzen et al, Integrative Biology, 2013).
  • Margination of late stage infected red blood cells.
  • Deformability based cell margination: Separation of late stage infected red blood cells (HW Hou et al, Lab-on-a-Chip, 2010).
  • Shear modulated inertial microfluidic biochip: High throughput separation of circulating tumor cells (AAS Bhagat et al, Lab-on-a-Chip, 2011).
  • High speed video (6400 fps) illustrating the complete isolation of MDA-MB-231cells from WBCs at the device outlet. Focused MDA-MB-231 cells (near the inner wall (top side)) are clearly distinguished from WBCs based on morphology and phase contrast. Few platelets are observed going into the CTC outlet. However, their presence do not interfere during counting using immunofluorescence staining or downstream molecular assay such as PCR. (ME Warkiani et al, Lab on a Chip, 2014).
  • High speed video (6400 fps) captured at the outlet of spiral biochip showing isolation of few CTCs from peripheral blood of a patient with advanced metastatic lung cancer. This movie clearly demonstrates the performance of our device for efficient enrichment of CTC from blood samples. (ME Warkiani et al, Lab on a Chip, 2014).
  • High speed video (6400 fps) captured at the outlet of spiral biochip showing isolation of few CTCs from peripheral blood of a patient with advanced metastatic lung cancer. This movie clearly demonstrates the performance of our device for efficient enrichment of CTC from blood samples. (ME Warkiani et al, Analyst, 2014).
  • CTChip detection & isolation of circulating tumor cells (SJ Tan et al, Biomed Microdev, 2009; SJ Tan et al, Biosensors & Bioelect, 2010).
  • Stem cells spreading on aligned electrospun nanofibers.
  • Stem cells migrating on aligned electrospun nanofibers.
  • New Frontiers, Episode 3, Channel i (28 April 2004).
  • Growing big ideas, Channel NewsAsia (08 Sept 2011).
  • Prof. Lim Chwee Teck on Microfluidics and Cancer (MBI).
  • Report on Clearbridge BioMedics, Channel News Asia (13 Mar 2013).
  • Why Engineering?, FoE, NUS (13 Sept 2013).
  • The Start-UP: ClearBridge BioMedics, Channel News Asia (24 Dec 2013).
  • Prof. Lim Chwee Teck talks about his scientific research, Joint IFOM-MBI Conference (July 2014).
  • Report on Liquid based Tactile Sensor, Channel News Asia (23 Sept 2015).
  • Invented in Singapore : A Dynamic Education, Channel News Asia (19 Feb 2016).