Visualizing the storage lesion in vivo

Since the pioneering demonstration of ex vivo preservation of red blood cells (RBCs) a century ago, blood storage has become an important medical procedure for emergency transfusion during surgery and anemia treatment. However, stored RBCs undergo biochemical, structural and functional changes, widely referred to as storage lesion. These changes have implications on the perfusion in the microcirculation, hence decreasing the effectiveness of the transfusion in restoring physiological conditions. In this project, with the ultimate aim of improving the efficacy of blood transfusion, the goal is to quantify the effects of storage lesion on the microcirculation using a Hyperspectral and Laser Speckle Contrast imaging system.