Single-cell Measurement of Red Blood Cell Oxygen Delivery Rate

Oxygen (O2) is supplied throughout the body by hemoglobin (Hb) bounded in red blood cells (RBCs). The ability of RBCs to deliver O2 has been routinely assessed by P50, defined as the partial pressure of O2 (PO2) required to saturate the Hb in RBCs to 50%. Although this defines the ability of RBCs to carry O2 under equilibrium states, it cannot determine the efficacy of O2 delivery in the dynamic blood flow, which could occur over the span of ~3s in the microcirculation. Therefore, in this study, we the goal is to develop a microwell array oxygen-sensing chip for measuring the rate of O2 delivery from RBCs at a single-cell level.