Functional sorting of human stem cell derived cells (in collaboration with Dr Chia Hung Chen, NUS)
Human pluripotent stem cells (hPSC)-derived cells are a key to realize more personalized prediction of drug responses in vitro. However, the practical translation of hPSC-derived cells for pre-clinical drug testing is currently hampered by low and variable tissue-specific functions of these cells. A lot of research has been focusing on enhancing the differentiation of hPSCs but there is little technological development on the purification of hPSC-derived cells to get cells with more consistent functions. We aspire to provide a solution to purify hPSC- derived cells based on their tissue-specific catalytic functions that have a direct relevance to in vitro drug testing assays. We are developing an integrated microfluidic system to measure the tissue-specific enzymatic activities of individual cells and sort them based on their enzymatic levels.
Directing lineage specification of hPSC-derived endothelial cells with substrate topography and shear stress
Pluripotent stem cell derived endothelial cells (PSC-EC) are an upcoming alternative endothelial cell (EC) source for various applications, but their arterial or venous specification is unclear. In this project, we are interested to evaluate biophysical cues, such as substrate topographies and fluid shear stress that can specify PSC-ECs into arterial or venous phenotypes. We aim to design a multiplexed microfluidic shearing device that can incorporate various topographical cues to screen for PSC-EC responses over a large range of physiological shear stresses (1-30 dynes/cm2).